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H0137 Sigma-Aldrich

HT Media Supplement (50×) Hybri-Max

lyophilized powder, γ-irradiated, BioXtra, suitable for hybridoma



Related Categories Cell Culture, Conditioned media supplements and reagents, Hybridoma Platform, Hybridoma Reagents, Reagents and Supplements,
Quality Level   200
sterility   γ-irradiated
product line   BioXtra
form   lyophilized powder
application(s)   cell culture | hybridoma: suitable
impurities   endotoxin, tested
storage temp.   −20°C



HT Media supplement is a cocktail hypoxanthine and thymidine qualified for use in hybridoma selection and cell culture applications. HT Media Supplement (50×) Hybri-Max has been used as a supplement in INS-1 β-cells co-culture medium. It is suitable for use as medium for hybridoma cells and as Dulbecco′s Modified Eagle Medium (DMEM) for HeLa S3 and MTH1-KO cells.


Reconstitute contents of vial with 10 ml sterile cell culture medium. Stock solution is sufficient to prepare 500 ml medium. Final working concentration: 100 μM hypoxanthine, 16 μM thymidine.

Legal Information

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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