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H0164 Sigma-Aldrich

Anti-Histone H3 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti H3 Antibody, Histone H3 Antibody - Anti-Histone H3 antibody produced in rabbit, Histone H3 Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:250-1:500 using mouse fibroblast NIH3T3 cells
  western blot: 1:2,500-1:5,000 using whole cell extract of the rat pheochromocytoma PC12 cell line
  western blot: 1:5,000-1:10,000 using whole cell extract of the A431 human epidermoid carcinoma cell line or a whole cell extract of the mouse fibroblast NIH3T3 cell line
species reactivity   mouse, plant, human, Drosophila, rat, chicken, Xenopus
mol wt   antigen mol wt 17 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P68431
Gene Information   human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)

Description

General description

Histone proteins are known to stabilize and regulate the nuclear DNA. Core histones, H2A, H2B, H3 and H4 aid the packaging of eukaryotic nuclear DNA into repeating nucleosome units while the linker histone, H1, binds to DNA between the nucleosomes. A diverse and elaborate array of post-translational modifications, including acetylation, phosphorylation and methylation, ubiquitination and ADP ribosylation occur in histones, particularly on the N-terminal tail domains of histones, particularly of H3 and H4.These modifications dictate chromatin states and the gene expression.
Phosphorylation of H3, mainly on Ser10 and Ser28, is localized to a small fraction of highly acetylated H3 and occurs primarily in response to mitogenic and stress stimuli. Phosphorylation of Ser10 is considered a crucial event for the onset of mitosis. It is tightly correlated with chromosome condensation during both mitosis and meiosis. Histone methylation, like acetylation, is a complex, dynamic process involved in a number of processes, including transcriptional regulation, chromatin condensation, mitosis ad heterochromatin assembly. Conserved lysine residues in the N-terminal tail domains of histone H3, Lys4, Lys9, and Lys27 are the preferred sites of methylation. Histone lysine residues can be mono-, di-, and tri-methylated at different heterochromatic subdomains, adding further complexity to the regulation of chromatin structure.

Specificity

Anti-Histone H3 specifically recognizes histone H3.

Immunogen

synthetic peptide corresponding to amino acids 125-136 located at the C-terminus of human histone H3, conjugated to KLH. This sequence is identical in many species including rat, mouse, chicken, Xenopus, Drosophila, and plant histone H3.

Application

Anti-Histone H3 antibody produced in rabbit has been used in immunoblotting and immunofluorescence microscopy.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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H9908 Monoclonal Anti-phospho-Histone H3 (pSer28) antibody produced in rat, ~0.5 mg/mL, clone HTA28, purified immunoglobulin, buffered aqueous solution
H9161 Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
H9286 Anti-acetyl-Histone H3 (Ac-Lys9) antibody produced in rabbit, 2-2.5 mg/mL, affinity isolated antibody, buffered aqueous solution
H0412 Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
H0788 Monoclonal Anti-acetyl- & phospho-Histone H3 (Ac-Lys9, pSer10) antibody produced in mouse, ~2 mg/mL, clone APH3-64, purified immunoglobulin, buffered aqueous solution
H0913 Anti-acetyl-Histone H3 (Ac-Lys9) antibody, Mouse monoclonal, clone AH3-120, purified from hybridoma cell culture
D5567 Anti-dimethyl-Histone H3 (diMe-Lys9) antibody produced in rabbit, IgG fraction of antiserum, buffered aqueous solution
M4819 Anti-methyl-Histone H3 (Me-Lys4) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution

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