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H0267 Sigma

Hemoglobin A0, Ferrous Stabilized human

lyophilized powder



Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, Hemoglobin, Plasma & Blood Proteins,
form   lyophilized powder
solubility   H2O: soluble 20 mg/mL
suitability   suitable for electrophoresis and chromatography standard (has not been tested for functional equivalence against native preparations (unlyophilized ferrous hemoglobins).)
storage temp.   −20°C
Gene Information   human ... HBB(3043)



Package size indicates the amount of hemoglobin as determined by the procedure of Drabkin, D.L., J. Biol. Chem., 164, 703 (1946).


When reconstituted with buffer, gives >85% ferrous hemoglobin.


Hemoglobin A0 was used to see how coronary intraplaque hemorrhage evokes a novel atheroprotective macrophage phenotype. It was used in the determination of fetal hemoglobin by time-resolved immunofluorometric assay.

General description

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
What is the structure of Product H0267, Hemoglobin A0?
Hemoglobin A0 is the major hemoglobin protein of adult hemoglobin. It is a tetrameric protein consisting of 2 alpha and 2 beta chains, each of which contains a heme moiety and iron.
What is the molecular weight of H0267 Hemoglobin A0?
Sigma has not evaluated the molecular weight of hemoglobin.  Based on the reported sequence and including the weight of the 4 heme groups and 4 iron atoms, the molecular weight is approximately 64,677 Daltons. The Structure and Action of Proteins, p. 52, Harper and Row (1969).
How can Product H0267, Hemoglobin A0, be dissolved?
Hemoglobin is soluble in water at 20 mg/mL.  Solutions in phosphate buffer at about neutral pH can also be prepared.
What is the solution stability of Product H0267, Hemoglobin A0?
Solutions of ferrous hemoglobin are not stable indefinitely due to the likely autoxidation of the heme group leading to the formation of the ferric derivative (methemoglobin). It is recommended to prepare solutions fresh in deaerated water or buffer.  If necessary, store such prepared solutions no more than 1-2 days at 2-8°C.
Is there methemoglobin in Product H0267, Hemoglobin A0?         
The product may contain a small amount of methemoglobin as described on the specification sheet on our website:   http://www.sigmaaldrich.com/catalog/DataSheetPage.do?brandKey=SIGMA&symbol=H0267
Can Product H0267, Hemoglobin A0, be used in functional studies involving the binding and release of oxygen as a ligand?
Sigma has not validated this hemoglobin for use in functional studies. The iron in the heme moiety is in the +2 state.  However, this does not mean that this hemoglobin will behave necessarily like native hemoglobin nor be useful for functional studies of the molecule.
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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