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H0396 Sigma-Aldrich

Hydrocortisone-Water Soluble

BioReagent, suitable for cell culture

Synonym: Cyclodextrin-encapsulated hydrocortisone



Related Categories Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Corticosteroid, Cytokines, Growth Factors and Hormones,
Quality Level   200
biological source   plant (wild yam)
product line   BioReagent
form   crystalline
application(s)   cell culture | mammalian: suitable
solubility   H2O: 400 mg/mL
shipped in   ambient
storage temp.   room temp
SMILES string   C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23
InChI   1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
Gene Information   human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)


General description

Hydrocortisone for use in epithelial and endothelial adherent cell culture applications.


100 mg in poly bottle

Package size based on hydrocortisone

Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do I calculate the molarity of product H0396, Hydrocortisone-Water Soluble?
ProductH0396 (Hydrocortisone-Water Soluble)is a complex of Hydrocortisone and cyclodextrin. Cyclodextrin is used to aid in the solubility of Hydrocortisone into water. In order to prepare a molar solution of Hydrocortisone you will have to base it on the Hydrocortisone content in the solution and not the entire complex. ProductH0396 is formulated to contain approximately85mg Hydrocortisone per gram of powder. Based onthis average,if you are preparing a 400 mg/ml stock solution, there will be approximately34 mg of Hydrocortisonein 1 ml. Below is the calculated molarity ofHydrocortisone when the complex is dissolved at 400 mg/ml in water.molecular weightX unknown molarity X volume = complex weight X complex ratio362.46g/mol X Molarity X 1 ml = 400 mg X 0.085 Molarity= 0.094 MHydrocortisonemolecular weight =362.46 g/molmolarity (unknown) = mol/Lvolume = 1mlcomplexweight = 400 mgcomplex ratio =85 mg Hydrocortisone/1 gm complex
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