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H0412 Sigma-Aldrich

Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-H3S10p, PH3 Antibody - Anti-phospho-Histone H3 (pSer10) antibody produced in rabbit, Ph3 Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   indirect immunofluorescence: 1:500 using human epitheloid carcinoma HeLa cell line treated with nocodazole.
  microarray: suitable
  western blot: 1:2,000 using whole cell extract of human acute T cell leukemia Jurkat cell line treated with nocodazole.
species reactivity   frog, human, mouse, Drosophila, rat, Caenorhabditis elegans, bovine, chicken
mol wt   antigen mol wt 17 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P68431
Gene Information   human ... H3F3A(3020), H3F3B(3021), HIST1H3A(8350), HIST1H3B(8358), HIST1H3C(8352), HIST1H3D(8351), HIST1H3E(8353), HIST1H3F(8968), HIST1H3G(8355), HIST1H3H(8357), HIST1H3I(8354), HIST1H3J(8356), HIST2H3A(333932), HIST2H3C(126961), HIST3H3(8290)
mouse ... H3f3a(15078), H3f3b(15081), Hist1h3a(360198), Hist1h3b(319150), Hist1h3c(319148), Hist1h3d(319149), Hist1h3e(319151), Hist1h3f(260423), Hist1h3g(97908), Hist1h3h(319152), Hist1h3i(319153), Hist2h3b(319154), Hist2h3c1(15077), Hist2h3c2(97114)
rat ... H3f3b(117056)

Description

General description

Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody recognizes histone H3 phosphorylated on Ser10 (17 kDa). Staining of pSer10-histone H3 in immunoblotting is specifically inhibited with the [pSer10] histone H3 immunizing peptide (human, amino acids 7-20). No inhibition is observed with the respective non-phosphorylated histone H3 peptide histone H3 (human, amino acids 7-20).

Immunogen

synthetic, phosphorylated peptide (pSer10) histone H3 (amino acids 7-20) corresponding to the N-terminus of human histone H3. The sequence is identical in many species.

Application

Rabbit polyclonal anti-phospho-Histone H3 (pSer10) antibody is used to tag Ser10 phosphorylated histone H3 for detection and quantitation by immunocytochemical and immunohistochemical (IHC) techniques such as immunoblotting and immunofluorescence. It is used as a probe to determine the presence and roles of Ser10 phosphorylated histone H3 in chromosome condensation during both mitosis and meiosis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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