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  • H1029 - Monoclonal Anti-polyHistidine antibody produced in mouse

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H1029 Sigma-Aldrich

Monoclonal Anti-polyHistidine antibody produced in mouse

clone HIS-1, ascites fluid

Synonym: Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal Anti-polyHistidine, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

  •  NACRES NA.56

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies and Conjugates, Antibodies to Peptide and Protein Fusion Tags, HF-HI,
conjugate   unconjugated
clone   HIS-1, monoclonal
biological source   mouse
application(s)   dot blot: suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 1:3,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein
form   (Suppled as ascites fluid with 15mM sodium azide.)
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG2a
antibody product type   primary antibodies
Quality Level   200

Description

General description

The Monoclonal Anti-polyHistidine (mouse IgG2a) antibody recognizes native or denatured, reduced forms of synthetic polyhistidine and polyhistidine-tagged fusion proteins. The antibody preferentially recognizes the N-terminal tagged fusion protein and is reactive with fusion proteins expressed by the prokaryotic expression vectors pET, pRSET, and pTrc.

Specificity

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Immunogen

recombinant polyHistidine tagged fusion protein.

Application

Antibody suitable for immunoblotting, dot blot assays, ELISA and immunocytochemistry. Monoclonal Anti-polyHistidine antibody produced in mouse has been used in Western blotting.

Legal Information

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

Biochem/physiol Actions

The IgG2a (Immunoglobulin G 2a) gene actively takes part in stimulating autoimmunity in mice. The gene is found to be highly expressed in virus infection.

Other Notes

Recommended dilution for immunoblotting is 1:3000

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How many histidines are required for proper recognition by Product H1029, Monoclonal Anti-polyHistidine antibody?
Product H1029, Monoclonal Anti-polyHistidine antibody requires at least 6 histidines in a row for recognition.
What is the detection limit of Product H1029, Monoclonal Anti-polyHistidine antibody?
The detection limit of product H1029 is 0.5 μg/ml or less.
Is Product H1029, Monoclonal Anti-polyHistidine antibody, suitable for use in immunofluorescence studies?
Product H1029 has been used in immunofluorescence and a good picture of the staining has been published. Ker R. Marshall, et al., Journal of General Virology 83, 1601-1612 (2002).
My question is not addressed here, how can I contact Technical Service for assistance?
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Protocols & Articles

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Protocols

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Peer-Reviewed Papers
15

References

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