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H1136 Sigma-Aldrich

Hyaluronidase from Streptomyces hyalurolyticus

lyophilized powder

Synonym: Hyaluronate Lyase from Streptomyces hyalurolyticus

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Description

General description

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Application

Hyaluronidase from Streptomyces hyalurolyticus has been used:
• in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
• as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Biochem/physiol Actions

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GalNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.

Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How should Product H1136, Hyaluronidase, be reconstituted?
Prior to determining the activity of this enzyme, we reconstitute it in 20 mM sodium phosphate buffer, with 77 mM sodium chloride, 0.01% BSA, pH 7.0.
What amount of product is present in the vial of Product H1136, Hyaluronidase?
The dry weight of the product is not determined. The vial should contain a minimum of 300 units of enzyme.
What is the molecular weight of Product H1136, Hyaluronidase?
The molecular weight for hyaluronidase from Streptomyces hyalurolyticus has been reported to be 91,000DALTONS as determined by gel filtration.
Are any other components present in Product H1136, Hyaluronidase, besides the enzyme?
No protein or BSA has been added as a stabilizer. The enzyme was lyophilized from 0.5 mM Tris buffer, pH 7.0.
How is the activity of Product H1136, Hyaluronidase,determined?
Sigma assays the enzyme against a USP hyaluronidase reference standard as described in USP XXII-NF XVII, 644 (1990).
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles

Articles

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Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure is a turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) of Hyaluronidase activity. It is based on the following reaction:
Keywords: Extinction coefficient

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Peer-Reviewed Papers
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References

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