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H17001 Sigma-Aldrich

Wnt3A, human

recombinant, expressed in HEK 293 cells human

Synonym: Wnt-3A, Wnt

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements
recombinant   expressed in HEK 293 cells human
assay   >90% (SDS-PAGE)
form   powder
potency   ≤20 ng/mL EC50
mol wt   mol wt 38-42 kDa
impurities   <1 EU/μg endotoxin
storage temp.   −70°C

Description

Analysis Note

The activity was determined by dose dependent Alkaline Phosphatase production in MC3T3 E1 mouse preosteoblast cells and stabilization of beta catenin in mouse L929 cells.

General description

Wnt3A is a member of the Wnt gene family that consists of structurally linked genes that encode secreted signaling proteins.

Reconstitution

It is recommended to reconstitute the protein to ≤100μg/mL in sterile 1X PBS containing 0.1% endotoxin-free HSA.

Physical form

Lyophilized from a 0.2 μm filtered solution containing 0.064 mg/mL protein in 1X PBS pH 7.4 + 500mM NaCl + 1% CHAPS.

Storage and Stability

Upon reconstitution, the cytokine can be stored at 4°C for short term or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

Protocols & Articles

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