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  • H17002 - LIF (Leukemia Inhibitory Factor), human

H17002 Sigma-Aldrich

LIF (Leukemia Inhibitory Factor), human

recombinant, expressed in HEK 293 cells



Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements
recombinant   expressed in HEK 293 cells
assay   >95% (SDS-PAGE)
potency   ≤0.2 ng/mL EC50
mol wt   33-48 kDa (glycosylated)
impurities   <1 EU/μg endotoxin
storage temp.   −20°C


General description

LIF promotes long-term maintenance of embryonic stem cells by suppressing spontaneous differentiation.


Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1X PBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Storage and Stability

Upon reconstitution, the cytokine can be stored at 4°C for short term only or at -20°C to -80°C in aliquots for long term. Avoid repeated freeze-thaw cycles.

Analysis Note

The activity was determined by the dose- dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis

Protocols & Articles

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