H1758 Sigma-Aldrich

Hydrochloric acid

36.5-38.0%, BioReagent, for molecular biology

  • CAS Number 7647-01-0

  • Empirical Formula (Hill Notation) HCl

  • Molecular Weight 36.46

  •  Beilstein/REAXYS Number 1098214

  •  EC Number 231-595-7

  •  MDL number MFCD00011324

  •  PubChem Substance ID 24895478

  •  NACRES NA.31



Related Categories Acids, Acids & Bases, Biochemicals, Chemical Synthesis, Core Bioreagents,
Quality Level   200
grade   for molecular biology
vapor density   1.3 (vs air)
vapor pressure   3.23 psi ( 21.1 °C)
  7.93 psi ( 37.7 °C)
product line   BioReagent
assay   36.5-38.0%
form   liquid
concentration   30-50%
color   light yellow
pH   < 1 (20 °C)
bp   >100 °C (lit.)
solubility   H2O: soluble
density   1.2 g/mL at 25 °C (lit.)
cation traces   Fe: ≤0.2 ppm
  heavy metals (as Pb): ≤1 ppm
suitability   suitable for molecular biology
SMILES string   Cl
InChI   1S/ClH/h1H



Hydrochloric acid has been used:
• for the solubilization of formazan crystals
• to adjust pH in various buffers
• for the preparation of giant reed extract

Hydrochloric acid has been used:
• in cell viability assays
• for DNA denaturation
• for pH adjustments in sucrose hydrolysis experiment


100, 500 mL in glass bottle

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Target organs 
Respiratory system
UN 1789 8 / PGII
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How do I calculate the molarity of Product No. H1758, Hydrochloric acid?
The molarity or molar concentration can be calculated using the following formula: [Density x 1000 x concentration (%)] / (molecular weight)
What is the molarity or molar concentration of Product No. H1758, Hydrochloric acid?
Based on an approximate density of 1.2 g/ml and a percentage range of 36.5 - 38.0%, concentrated HCl is in the range 11.6-12.0 M (which is also 11.6-12.0 Normal, for this monoprotic acid).
Is Product No. H1758, Hydrochloric acid, given a grade designation?
This product is designated as Molecular Biology grade and is suitable for molecular biology applications.
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Protocols & Articles


Enzymatic Assay of Catalase (EC

The continuous spectrophotometric rate reduction determination (A240, Light path = 1 cm) is based on the following reaction:
Keywords: Reductions

Enzymatic Assay of Ficin (EC

The spectrophotometric stop rate detemination (A280, Light path = 1 cm) is based on the following reaction:

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