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  • H1775 - Monoclonal Anti-Heat Shock Protein 90 antibody produced in mouse

H1775 Sigma-Aldrich

Monoclonal Anti-Heat Shock Protein 90 antibody produced in mouse

clone AC-16, ascites fluid



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Stress, Antibodies to Chaperonins and Heat Shock Proteins,
conjugate   unconjugated
clone   AC-16, monoclonal
biological source   mouse
application(s)   microarray: suitable
  western blot: 1:1,000 using cultured human foreskin fibroblasts
species reactivity   Sf9 cell line, rat, wheat germ, rabbit, human, mouse, chicken, Achlya
mol wt   antigen 88 kDa
shipped in   dry ice
storage temp.   −20°C
packaging   antibody small pack of 25 μL
antibody form   ascites fluid
isotype   IgG2b
Quality Level   200
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P07900
Gene Information   human ... HSP90AA1(3320), HSP90AB1(3326)
mouse ... Hsp90aa1(15519), Hsp90ab1(15516)


General description

HSP90AB1 (heat shock protein 90α family class B member 1) belongs to the Hsp90 family. It consists of major members-Hsp90α, Hsp90β, GRP94 (glucose-regulated protein 94) and Hsp75. It is located on chromosome 6p21. HSP90AB1is an ATP-dependent molecular chaperone, having a molecular weight of 90kDa. HSP90AA1 is located on human chromosome 14q32.31.


The antibody is reactive with both the constitutive and the inducible forms of HSP90. However, it does not bind to the native form of HSP90. It does not recognize HSP90 from E. coli and yeast.


heat shock protein 90 (HSP90) from the water mold Achlya ambisexualis


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Monoclonal Anti-Heat Shock Protein 90 antibody has been used in ovarian histomorphology, immunohistochemistry and western blotting.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

HSP90AB1 (heat shock protein 90α family class B member 1) participates in signal transduction, protein folding and degradation, and morphological evolution. It is required for the transport of client proteins between the cytoplasm and nucleus. In lung adenocarcinoma patients, overexpression of HSP90AB1 in non-small cell lung cancer tissues results in imperfect clinical predictions. Suppression of this gene reduces the potential of endothelial cells to form tube structures.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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