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H2500 Sigma-Aldrich

Hemoglobin from bovine blood

lyophilized powder

Synonym: Bovine hemoglobin, Hb, Methemoglobin

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Properties

Related Categories Application Index, Biochemicals and Reagents, Cell Biology, Cell Signaling Enzymes, Cell Signaling and Neuroscience,
Quality Level   200
biological source   bovine blood
form   lyophilized powder
mol wt   Mr ~64500
application(s)   MALDI-TOF: suitable
storage temp.   2-8°C
Gene Information   cow ... HBA1(281221), HBB(280813), HBE2(513240), HBE4(513108), HBG(511735), alpha globin(512439)

Description

General description

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with each 141 and 146 amino acids, respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.

Application

Hemoglobin from bovine blood has been used in:
• standard curve generation for the quantification intraparenchymal hemorrhage and parenchymal hemorrhage in spinal cord homogenate using Drabkin′s assay, Quadrupole-Ion Mobility-Time-of-Flight mass spectrometery
•  the generation of molecularly imprinted polymers (MIPs) to mimic high molecular-weight polyethylene glycol (PEG) in crystallization studies

The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Packaging

1, 5, 10, 25 g in poly bottle

Biochem/physiol Actions

Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide. Bovine hemoglobin is used in the generation of hemoglobin-vesicles (HbV) for oxygen transport. It is thermally stable and displays high affinity to oxygen when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine haemoglobin is recommended for treating autoimmune hemolytic anemia.

Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Caution

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX
Keywords: Chromatography, High performance liquid chromatography

Peer-Reviewed Papers
15

References

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