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H2625 Sigma

Hemoglobin from bovine blood

suitable for protease substrate, substrate powder

Synonym: Bovine hemoglobin, Hb, Methemoglobin



Related Categories Application Index, Biochemicals and Reagents, Enzymes, Inhibitors, and Substrates, General Protease Substrates, Hemoglobin,
form   substrate powder
mol wt   Mr ~64500
solubility   H2O: soluble 20 mg/mL
suitability   suitable for protease substrate
storage temp.   2-8°C
Gene Information   cow ... HBA1(281221), HBB(280813), HBE2(513240), HBE4(513108), HBG(511735), alpha globin(512439)



The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.


Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.


1 kg in poly drum

25, 100 g in glass bottle

Preparation Note

Prepared from washed, lysed and dialyzed erythrocytes.

Biochem/physiol Actions

Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

General description

Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.

Price and Availability

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Cellular Derived Blood Proteins and Enzymes

Cell damage is induced by reactive oxygen species (ROS). ROS are either free radicals, reactive anions containing oxygen atoms, or molecules containing oxygen atoms that can either produce free radic...
BioFiles 2006, 1.5, 4.
Keywords: Aerobic, Alzheimer Disease, Bacterial conjugations, Biofiles, Cancer, Metabolism, Oxidations, Parkinson Disease, Reductions, Respiration

HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Hemoglobin Variants on Discovery® BIO PolyMA-WAX
Keywords: Chromatography, High performance liquid chromatography


Enzymatic Assay of Pepsin (

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Enzymatic Assay of Proteinase K (EC

This procedure may be used for determination of Proteinase K activity using hemoglobin as the substrate. It is not used to assay the activity of immobilized Proteinase K products, such as Catalog Num...
Keywords: Immobilization

Enzymatic Assay of Proteinase K with Hemoglobin Substrate

The objectiveis to standardize a procedure for the enzymatic determination of Proteinase K using Hemoglobin as the substrate.
Keywords: Filtration

Peer-Reviewed Papers


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