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  • H3034 - Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit

H3034 Sigma

Anti-Histone Deacetylase 3 (HDAC3) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Epigenetics,
species reactivity   rat, human, mouse
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using human lymph node sections
  immunoprecipitation (IP): 1:2,500 using whole lysate of NIH3T3 cells
  microarray: suitable
  western blot: 1:10,000 using nuclear extract from HeLa human epithelioid carcinoma cells
  western blot: 1:2,000 using whole extract of PC-12 rat pheochromocytoma cell line
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 50 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HDAC3(8841)
mouse ... Hdac3(15183)
rat ... Hdac3(84578)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 411-428 of human and chicken HDAC3.

General description

Histone acetylation is a dynamic process whose levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Six or seven different mammalian HDACs have been described. HDAC1, HDAC2 and HDAC3 are similar to yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are similar to yeast Hda1 protein. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosomal condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Physical form

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.


Anti-Histone Deacetylase 3 specifically recognises histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC3 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC3 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding. The antibody reacts with HDAC3 of human, rat, and mouse origin.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunoprecipitation (1 paper)

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