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  • H3159 - Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

H3159 Sigma-Aldrich

Anti-Histone Deacetylase 2 (HDAC2) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Epigenetics,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500 using protease-digested, human lymph node sections
  immunoprecipitation (IP): 50 μg using whole lysate of NIH3T3 cells
  microarray: suitable
  western blot: 1:20,000 using nuclear extract from human HeLa cells
species reactivity   mouse, human
mol wt   antigen 55 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q92769
Gene Information   human ... HDAC2(3066)
mouse ... Hdac2(15182)


General description

Histone Deacetylase (HDACs) have been described as six or seven different groups in mammals. HDAC1, HDAC2 and HDAC3 are like yeast Rpd3 protein, while HDAC4, HDAC5 and HDAC6 are like yeast Hda1 (Histone Deacetylase 1) protein.


Anti-Histone Deacetylase 2 specifically recognizes histone deacetylase 2 by immunoblotting and immunoprecipitation (55 kDa). An additional band of lower molecular weight may be detected in some cell line extracts by immunoblotting. Staining of HDAC2 by immunoblotting is specifically inhibited with the immunizing peptide. The product is also useful for the detection of HDAC2 by immunohistochemistry. The epitope(s) recognized by the antibody is resistant to routine formalin-fixation and paraffin embedding. The antibody reacts with HDAC2 of human, rat and mouse origin.


synthetic peptide (C-SGEKTDTKGTKSEQLSNP) corresponding to the C-terminus region of histone deacetylase 2 of human origin (amino acids residues 471-488 with N-ternimal added cysteine) conjugated to maleimide-activated KLH. This sequence is highly conserved in mouse and chicken (2 amino acids and 1 amino acid substitutions, respectively).


Anti-Histone Deacetylase 2 (HDAC2) antibody has been used:
• in immunoblotting and dot blot
• in immunoprecipitation
• in immunohistochemistry
• in enzyme linked immunosorbent assay (ELISA)
• in western blotting
• in chromatin immunoprecipitation(ChiP)

Physical form

Solution in 0.01 M phosphate bufffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Histone acetylation is a dynamic process whose levels are determined by the net activities of Histone acetyl transferase (HATs) and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Histone deacetylases activities were often, but not always, associated with transcriptional repression and nucleosome condensations. HDAC1, HDAC2 and several other HDACs are the catalytic subunits of different multiprotein regulatory complexes.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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