H3375 Sigma-Aldrich


≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895572

  •  NACRES NA.25



Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
Quality Level   300
assay   ≥99.5% (titration)
form   crystalline powder
application(s)   cell culture | mammalian: suitable
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
solubility   water: 500 mg/mL, clear, colorless
Featured Industry   Diagnostic Assay Manufacturing
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)


General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.


HEPES has been used:
• As a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
• As a component of Hanks’ solution used in the degradation testing of Iron
• As a component of HEPES medium in the transfection of cell culture with plasmids
• Along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
• As a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies


1 kg in poly bottle

25, 100, 250, 500 g in poly bottle

5, 25, 50 kg in poly drum

Other Notes

Go to Redi-Dri Product Listing RDD002
Easily compare specifications for HEPES products with the HEPES specification table.

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the purpose of using HEPES in cell culture?
HEPES provides supplemental buffering for media in pH range of 7.2 to 7.6. It has no nutritional value to cells.
What is the concentration of HEPES used in cell culture?
HEPES is typically used in cell culture in the range of 10-25 mM.
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