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H3375 Sigma

HEPES

≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Properties

Related Categories Apoptosis and Cell Cycle, Biochemicals and Reagents, Biological Buffers, Buffers, Buffers A to Z,
Quality Level   ELITE
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
assay   ≥99.5% (titration)
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
Featured Industry   Diagnostic Assay Manufacturing

Description

Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Packaging

1 kg in poly bottle

25, 100, 250, 500 g in poly bottle

5, 25, 50 kg in poly drum

Legal Information

Redi-Dri is a trademark of Sigma-Aldrich Co. LLC

Other Notes

Go to Redi-Dri Product Listing RDD002

Protocols & Applications

Ensure correct concentrations of buffer solutions with the Buffer Calculator

Price and Availability


Added Consistency

Bulk Chemicals

Webinar: Biological Buffers
Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TL6809000

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
What is the purpose of using HEPES in cell culture?
HEPES provides supplemental buffering for media in pH range of 7.2 to 7.6. It has no nutritional value to cells.
What is the concentration of HEPES used in cell culture?
HEPES is typically used in cell culture in the range of 10-25 mM.
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Protocols & Articles

Articles

3D Organoid Culture: New In Vitro Models of Development and Disease

Model systems drive biological research by recapitulating body processes and functions from the molecular to whole organism level. The human body is composed of both cellular and non-cellular materia...
Keywords: Adhesion, Cell attachment, Cell culture, Cryopreservation, Diseases, Growth factors, Infectious Diseases, Processes and Functions, Vitamins

Related Content

BioUltra Biological Buffers

A buffer, as defined by Van Slyke [1], is "a substance which by its presence in solution increases the amount of acid or alkali that must be added to cause unit change in pH". Buffers are thus very i...
Keywords: Absorption, Adsorption, Biochemistry, Biological Buffers, Biological processes, Buffers, PAGE

Buffer Reference Center

Citric Acid – Na2HPO4 Buffer Solutions, pH approx. 2.6–7.61 Citric acid monohydrate, C6H8O7 • H2O, M. wt. 210.14; 0.1M-solution contains 21.01 g/l. Na2HPO4, M. wt. 141.98; 0.2M-solution contains 28.4...
Keywords: Biological Buffers, Buffers

Peer-Reviewed Papers
15

References

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Product #

Description

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T1503 Trizma® base, Primary Standard and Buffer, ≥99.9% (titration), crystalline

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