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H3537 Sigma-Aldrich

HEPES solution

BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging,
grade   BioPerformance Certified
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
sterility   0.2 μm filtered
concentration   1 M
impurities   Bioburden, tested
  DNase, RNase, Protease, Nickase, free
  endotoxin, tested
useful pH range   6.8 - 8.2
cation traces   Fe: <5 ppm
  heavy metals (as Pb): <5 ppm
suitability   suitable for cell culture
  suitable for molecular biology
Featured Industry   Diagnostic Assay Manufacturing

Description

Application

RNAse HEPES has been used:
• To supplement Dulbecco’smodified Eagle’smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

Certificate of Origin


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Protocols & Articles

Articles

Attachment-Dependent Cell Growth and Cell Proliferation in the MultiScreen Assay System

Numerous bioassays utilize cell cultures to quantitate the effects of potential growth modifiers. These assays are typically performed in 96-well microwell plates for “convenience” and are usually fo...
Keywords: Adhesion, Cell attachment, Cell culture, Cell proliferation, Culture media, Filtration, Growth factors, Scintillation, Transduction

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Peer-Reviewed Papers
15

References

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