H3537 Sigma-Aldrich

HEPES solution

BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Synonym: N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)



Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging,
Quality Level   200
grade   BioPerformance Certified
sterility   0.2 μm filtered
concentration   1 M
application(s)   cell culture | mammalian: suitable
impurities   Bioburden, tested
  DNase, RNase, Protease, Nickase, free
  endotoxin, tested
useful pH range   6.8 - 8.2
cation traces   Fe: <5 ppm
  heavy metals (as Pb): <5 ppm
suitability   suitable for molecular biology
Featured Industry   Diagnostic Assay Manufacturing


General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.


RNAse HEPES has been used:
• To supplement Dulbecco’smodified Eagle’smedium for maintenance of cell line and RPMI medium to wash rat islets
• To recover purified ribonucleotide
• As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
• As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles


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Keywords: Adhesion, Cell attachment, Cell culture, Cell proliferation, Culture media, Filtration, Growth factors, Scintillation, Transduction

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