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H3662 Sigma-Aldrich

HEPES sodium salt solution

1M, BioReagent, suitable for cell culture

  • CAS Number 75277-39-3

  • Empirical Formula (Hill Notation) C8H17N2NaO4S

  • Molecular Weight 260.29

  •  MDL number MFCD00036463

  •  PubChem Substance ID 24895590

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffer Convenience Packaging, Buffer Solutions,
InChI Key   RDZTWEVXRGYCFV-UHFFFAOYSA-M
sterility   sterile-filtered (0.2 μm filtered)
product line   BioReagent
impurities   Bioburden, tested
  DNase, RNase. Protease, Nickase, free
  endotoxin, tested
  <5 ppm Heavy metals (as Pb)
cation traces   Fe: <5 ppm
suitability   suitable for cell culture

Description

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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