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H4034 Sigma-Aldrich

HEPES

BioPerformance Certified, ≥99.5% (titration), cell culture tested

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895616

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Properties

Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions,
Quality Level   ELITE
grade   BioPerformance Certified
InChI Key   JKMHFZQWWAIEOD-UHFFFAOYSA-N
assay   ≥99.5% (titration)
impurities   endotoxin and total aerobic microbial count, tested
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
cation traces   Fe: ≤5 ppm
suitability   cell culture tested
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, RNase, NICKase, protease, none detected

Description

General description

HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
• To supplement Dulbecco’s modified Eagle’s medium to culture and maintain cell lines
• As a component of platelet suspension buffer
• To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
• As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
• For the adjustment and maintenance of pH of biological solutions
• As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
• For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
• As a component of keratinocyte and fibroblast culture medium

Packaging

1 kg in poly bottle

100, 500 g in poly bottle

25 kg in poly drum

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
TL6809000
Protocols & Articles

Protocols

Immunoprecipitation Procedure

To search our entire portfolio of antibodies for your research needs, use our Antibody Explorer Tool.
Keywords: Buffers, Cell disruption, Centrifugation, Enzyme activity, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, PAGE, Size-exclusion chromatography, Western blot

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Peer-Reviewed Papers
15

References

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