H4034 Sigma-Aldrich


BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895616

  •  NACRES NA.25



Related Categories BioPerformance Certified Buffers, Biochemicals and Reagents, Biological Buffers, Buffer / Buffer Salts, Buffered Salt Solutions,
Quality Level   400
grade   BioPerformance Certified
assay   ≥99.5% (titration)
application(s)   cell culture | mammalian: suitable
impurities   endotoxin and total aerobic microbial count, tested
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
cation traces   Fe: ≤5 ppm
absorption   ≤0.05 at 260 in H2O at 0.1 M
  ≤0.05 at 280 in H2O at 0.1 M
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, RNase, NICKase, protease, none detected
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)


General description

HEPES buffer does not confer cytotoxic effects on cells and thus can be used in animal cell cultures.

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.


HEPES has been used:
• To supplement Dulbecco’s modified Eagle’s medium to culture and maintain cell lines
• As a component of platelet suspension buffer
• To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
• As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
• For the adjustment and maintenance of pH of biological solutions
• As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
• For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
• As a component of keratinocyte and fibroblast culture medium


1 kg in poly bottle

100, 500 g in poly bottle

25 kg in poly drum

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Buffers - From Bnech to Bulk

pHast Pack
Protocols & Articles


Immunoprecipitation Procedure

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Keywords: Buffers, Cell disruption, Centrifugation, Enzyme activity, Immobilization, Immunoprecipitation, Individual protein Immunoprecipitation, PAGE, Size-exclusion chromatography, Western blot

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