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H4040 Sigma-Aldrich

HydroMatrix Peptide Cell Culture Scaffold

24 well plate

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Description

Components

HydroMatrix is a peptide nanofiber 3-dimensional scaffold that promotes cell growth and migration. It utilizes specific peptides that self-assemble from fluid precursors into highly crosslinked peptide hydrogels when presented with increased temperatures or ionic strengths. By inducing a rapid transformation from solution to gel, a peptide nanofiber scaffold is generated. Adjusting the concentration of the HydroMatrix solution, researchers can control the flexibility of the 3-D architecture to meet their application′s specific needs.

Application

HydroMatrix has been shown to support improved growth and migration in the proliferation of many cell types, including neural stem cells, neurons, glia, astrocytes, fibroblasts, and keratinocytes.

Caution

The lyophilized product at -20°C and is stable for many years at this temperature. The hydrogel should be stored at -70°C to maximize its active span. The 1% (w/v) stock solution should be stored -20°C and is stable for 3-6 months.

Preparation Note

This product is supplied as a lyophilized powder. A 1% (w/v) stock solution at 10 mg/mL can be prepared with addition of sterile water, resulting in a rigid gel. A 0.5% (w/v) solution will lead to a softer gel that is more suitable for cell migration and angiogenesis. Lower concentrations in the range of 0.15%-0.25% are more suitable for many cell types including neurons. In aqueous solution, HydroMatrix has a pH of 2.5 and should not be added directly to cells without prior addition of a buffer or medium. Once the hydrogel has formed, do not attempt to mix the gel any further, as this risks destroying the hydrogel integrity. Manipulation of the cells should be carefully performed to avoid disruption of the scaffold.

Legal Information

HydroMatrix is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
How is HydroMatrix Peptide Scaffold, Product A4040, supplied?
This product is supplied in the plate in a lyophilized form.For the 24 well plate to achieve a 1% w/v (10 mg/ml stock concentration), reconstitute with H2O at a volume of 500 microliters per well.
How do I store HydroMatrix Peptide Scaffold, Product A4040?
Store the product at -20°C as supplied.Once the material is reconstituted, to a 1% (w/v) (10 mg/ml) stock solution, with dH20, the stock solution should be stored at -20°C and is stable for 3-6 months.
How thick is the layer on the HydroMatrix Peptide Scaffold, Product A4040,plate?
We have not determined the actual thickness of the layer, but it is thick enough to coat the bottom of the well.
How doesHydroMatrix Peptide Cell Culture Scaffold compare to PuraMatrix from Becton-Dickenson?
Both products are peptide hydrogels.There is no similarity between the peptides used to create the matrices.They are both just classified as self-assembling peptide hydrogels. The sequence for the peptide is considered proprietary.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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