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H4397 Sigma-Aldrich

TiterMax® Classic Adjuvant


  •  NACRES NA.46



Related Categories Adjuvant Reagents, Adjuvant emulsions, Antibodies, Antibody Purification and Characterization, Cell Culture,
Quality Level   100
form   liquid
availability   not available in Japan
storage temp.   2-8°C


General description

The effectiveness of an oil emulsion vaccine greatly depends on the form and stability of the emulsion. TiterMax Classic is a water-in-oil formulation with up to 90% water and a powerful adjuvant used in many protocols. TiterMax Classic Adjuvant contains three essential ingredients: a block copolymer, CRL89-41, squalene (a metabolizable oil) and a unique microparticulate stabilizer. The emulsion is less viscous and suitable for injection through small needles.


For mice, four doses of 10 microl each into subcutaneous or muscular sites is recommended. TiterMax® was used in mice followed by isolation of CD4 T cells for epitope mapping and to test vaccines against Neisseria species.

TiterMax® Classic Adjuvant has been used to boost the production of antigen-specific polyclonal IgG in rabbits.

Biochem/physiol Actions

TiterMax Classic has been especially formulated with squalene to produce stable water-in-oil emulsions with up to 90% water. It is considerably easier to emulsify TiterMax Classic than Freund’s adjuvant. The resulting emulsion is less viscous, making it easier to inject through small needles.

Legal Information

TiterMax is a registered trademark of TiterMax USA, Inc


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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