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H4791 Sigma

Bone Morphogenetic Protein 2 human

BMP-2, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: BMP-2

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements, Recombinant Bone Morphogenetic Proteins (BMP),
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥90% (SDS-PAGE)
form   lyophilized powder
potency   1-5 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 30-38 kDa (glycosylated)
packaging   pkg of 10 μg
mfr. no.   Corning
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/μg Endotoxin level
suitability   suitable for cell culture
UniProt accession no.   P12643
storage temp.   −20°C
Gene Information   human ... BMP2(650)

Description

Analysis Note

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-2 in the ATDC-5 cell line (mouse chondrogenic cell line).

Packaging

10 μg in poly bottle

Preparation Note

This Bone Morphogenetic Protein 2 (BMP-2) is produced from a DNA sequence encoding the human BMP-2 protein, expressed in HEK 293 cells. It is a glycosylated homodimer linked by a single disulfide bond with an apparent molecular mass of 30-38 kDa.

Biochem/physiol Actions

It has been reported that Bone Morphogenetic Protein 2 (BMP-2) inhibits estradiol induced proliferation of human breast cancer cells. BMP-2 signaling mediates apoptosis by activation of the TAK1-p38 kinase pathway that is negatively regulated by Smad6. Cellular responses to BMP-2 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. One BMP type II receptor and two BMP type I receptors have been identified. Both BMP type I receptors bind BMP-2 with high-affinity in the absence of BMP receptor type II.

General description

Bone Morphogenetic Protein 2 is a member of the TGF-β superfamily of cytokines that affect bone and cartilage formation. It is important for skeletal development during embryogenesis. BMP-2 induces chondrocyte formation, osteoblast differentiation, and is involved in embryo dorsal-ventral patterning and organogenesis.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Articles

Injectable Hydrogels for Cell Delivery and Tissue Regeneration

Prathamesh M. Kharkar,1 April M. Kloxin1,2* 1Materials Science and Engineering and 2Chemical and Biomolecular Engineering University of Delaware, Newark, DE 19716 USA *Email: akloxin@udel.edu
Keywords: Addition reactions, Adhesion, Angiogenesis, Biochemistry, Biomaterials, Building blocks, Cardiovascular, Catalysis, Cell proliferation, Click chemistry, Clinical, Cycloadditions, Degradations, Growth factors, Reversed-phase chromatography

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Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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