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H4916 Sigma

Bone Morphogenetic Protein 4 human

BMP-4, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: BMP-4

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements, Recombinant Bone Morphogenetic Proteins (BMP),
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   2-10 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 34 kDa (glycosylated)
packaging   pkg of 10 μg
  pkg of 100 μg
  pkg of 1000 μg
mfr. no.   Corning
storage condition   avoid repeated freeze/thaw cycles
impurities   <1 EU/μg Endotoxin level
suitability   suitable for cell culture
UniProt accession no.   P12644
storage temp.   −20°C
Gene Information   human ... BMP4(652)

Description

Analysis Note

The specific activity was determined by its ability to induce alkaline phosphatase production in a dose response to BMP-4 in the ATDC-5 cell line (mouse chondrogenic cell line).

Packaging

10 μg in poly bottle

Preparation Note

This Bone Morphogenetic Protein-4 (BMP-4) is expressed in human HEK 293 cells as a glycosylated 34 kDa homodimer. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Mature human and mouse BMP-4 are 98% and 100% identical, respectively, to mature rat BMP-4 in their amino acid sequence.

Biochem/physiol Actions

BMPs create an environment conducive for bone marrow development by stimulating the production of specific bone matrix proteins and altering stromal cell and osteoclast proliferation. In addition to stimulating ectopic bone and cartilage development, BMPs may be an important factor in the development of the viscera, with roles in cell proliferation, apoptosis, differentiation, and morphogenesis. BMPs also appear to be responsible for normal dorsal/ventral patterning. BMP-4 specifies the development of ventral structures (e.g., skin from ectoderm and connective tissue/blood from mesoderm). Dorsal structures (nervous system and muscle) apparently appear when BMP-4 signals are interrupted through the activities of binding proteins. BMPs are found in tissues that induce bone or cartilage growth, such as demineralized bone and urinary epithelium.

Cellular responses to BMP-4 are mediated by the formation of hetero-oligomeric complexes of type I and type II serine/threonine kinase receptors, which play significant roles in BMP binding and signaling. Two BMP type I receptors and one BMP type II receptor have been identified. Both BMP type I receptors bind BMP-4 with high-affinity in the absence of BMP receptor type II.

General description

BMP are members of the TGF-β superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF-β family proteins, BMPs are highly conserved across animal species. Mature BMPs are 30-38 kDa proteins that assume a TGF-β-like cysteine knot configuration. Unlike TGF-β, BMPs do not form latent complexes with their propeptide counterparts. Most BMPs are homodimers, but bioactive natural heterodimers have been reported.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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