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H5041 Sigma-Aldrich

Cystatin C human

recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   0.3-1.5 μM IC50
quality   endotoxin tested
mol wt   dimer mol wt 12-13 kDa (non-glycosylated)
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/mg
suitability   suitable for cell culture
UniProt accession no.   P01034
storage temp.   −20°C
Gene Information   human ... CYTC(1471)

Description

Analysis Note

The inhibitory function of cystatin c on papain’s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC50 value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.

Packaging

10 μg in poly bottle

Preparation Note

This product is lyophilized from a PBS solution. IC50: 0.3-1.5 microM.

Application

Cystatin C is an inhibitor of cysteine proteases including cathepsin B (which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR).

General description

HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells and has an apparent molecular mass of 12-13 kDa. Cystatin C belongs to the cystatin superfamily. Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by elimination of small basic peptides or amino acids from the N-terminal end of protein. Cystatin C is used as a biomarker of kidney function. Recently, it has been studied for its role in predicting new-onset or deteriorating cardiovascular disease. It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease. Cystatin C was reported to antagonize transforming growth factor β signaling in normal and cancer cells.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

Protocols

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Peer-Reviewed Papers
15

References

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