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H5291 Sigma-Aldrich

Fibroblast Growth Factor-8b human

recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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Properties

Related Categories Cell Culture, FGF-4 to FGF-8, Fibroblast Growth Factors (FGF), Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   15-60 ng/mL ED50
mol wt   dimer mol wt 30-45 kDa (glycosylated)
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   endotoxin, tested
suitability   suitable for cell culture
storage temp.   −20°C

Description

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the BALB/3T3 cell line.

Packaging

10 μg in poly bottle

Preparation Note

HumanKine Fibroblast Growth Factor-8b, expressed in human HEK 293 cells is a glycosylated monomer with an apparent molecular mass of 30-45 kDa due to glycosylation. Production in human HEK 293 cells offers authentic glycosylation, which is absent when this cytokine is expressed in E. coli. Glycosylation contributes to stability in cell growth media and other applications.

Biochem/physiol Actions

FGF-8b belongs to the FGF protein family. FGF-8b is important in embryogenesis, in pathways that lead to left-right determination and coordinate the vertebral segmentation process, and in processes that activate Homeobox genes. There are several isoforms of FGF-8 produced by alternative splicing.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

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Cell Culture Guide
Protocols & Articles

Articles

Fibroblast Growth Factor Family (FGF)

Fibroblast Growth Factors (FGFs) are potent regulators of cell proliferation, differentiation and function and are critically important in normal development, tissue maintenance, wound repair and ang...
Keywords: Angiogenesis, Cancer, Cardiovascular, Cell proliferation, Diabetes, Diseases, Gene expression, Growth factors, Ligands

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Peer-Reviewed Papers
15

References

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