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H5388 Sigma-Aldrich

Hyaluronic acid sodium salt from rooster comb

avian glycosaminoglycan polysaccharide

Synonym: Poly(β-glucuronic acid-[1→3]-β-N-acetylglucosamine-[1→4]), alternating



Related Categories Biochemicals and Reagents, Biostabilization Agents, Carbohydrate, Carbohydrates, Carbohydrates A to Z,
Quality Level   200
biological source   chicken (rooster comb)
form   powder
storage temp.   −20°C
InChI   1S/C28H44N2O23.Na/c1-5(33)29-9-18(11(35)7(3-31)47-25(9)46)49-28-17(41)15(39)20(22(53-28)24(44)45)51-26-10(30-6(2)34)19(12(36)8(4-32)48-26)50-27-16(40)13(37)14(38)21(52-27)23(42)43;/h7-22,25-28,31-32,35-41,46H,3-4H2,1-2H3,(H,29,33)(H,30,34)(H,42,43)(H,44,45);/q;+1/t7-,8-,9-,10-,11-,12-,13+,14+,15-,16-,17-,18-,19-,20+,21+,22+,25-,26+,27-,28-;/m1./s1



Hyaluronic acid sodium salt from rooster comb has been used:
• as a component of bi-layer gelatin scaffold
• as a vehicle for release of gentamicin during left ear surgery in guinea pigs
• in hyaluronic acid binding assay in human embryonic kidney cell line 293T


1 g in poly bottle

100, 250 mg in poly bottle

Biochem/physiol Actions

High molecular mass polymer composed of repeating dimeric units of glucuronic acid and N-acetyl glucosamine which forms the core of complex proteoglycan aggregates found in extracellular matrix.

Hyaluronic acid is a polysaccharide present in the skin, connective tissue, vitreous humor and synovial fluid. It is essential for tissue hydration and functions to regenerate skin post injury or wounds. Hyaluronic acid, apart from having dermatologic and ophthalmologic applications, serves as a drug delivery vehicle. Hyaluronic acid also exhibits antioxidant and antiglycation properties.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the molecular weight of Hyaluronic acid, Product H5388?
Product No. H5388 is a linear heteropolysaccharide with repeating 3-O-(β-D-glucuronido)-N-acetyl-D-glucosamine units linked by β-(1-->4)) hexosaminidic bonds.The molecular weight of this subunit has not been determined, but could be calculated from the information above. Molecular weights of hyaluronic acids vary from 0.1-10 million, depending on the source.The hyaluronic acid from rooster comb is expected to have a molecular weight of 1-4 million.
What is the purity of Hyaluronic acid, Product H5388?
Sigma does not perform purity testing on this product due to the variable molecular weight and degradation that may occur in native glycosaminoglycans.This product is tested for activity and protein content which provide information regarding impurities and suitability.
What is the solubility of Hyaluronic acid, Product H5388?
For Sigma's analytical test, 15 mg of this product is dissolved in 5 mL of 0.3 M phosphate buffer, pH 5.35 at 25°C (i.e., 3 mg/mL).
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Protocols & Articles


Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
Vicki Caligur
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatography, Coagulation, Degradations, Enzyme-linked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, Help, High performance liquid chromatography, Inflammation, Ion-exchange chromatography, Ligands, Mass spectrometry, Methods, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain reaction, Polymerization reactions, Proteomics, Reductive aminations, Sequences, Spectroscopy, Sulfations, Transcription, Type, transformation

Glycosaminoglycans and Proteoglycans

Glycosaminoglycans (GAGs) are large linear polysaccharides constructed of repeating disaccharide units with the primary configurations containing an amino sugar (either GlcNAc or GalNAc) and an uroni...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Catalysis, Coagulation, Degradations, Growth factors, Inflammation, Ligands, Pentose phosphate pathway, Polymerization reactions, Sulfations


Enzymatic Assay of Hyaluronidase (

This procedure is a turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) of Hyaluronidase activity. It is based on the following reaction:
Keywords: Extinction coefficient

Peer-Reviewed Papers


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