H5416 Sigma-Aldrich

FLT3 Ligand human

recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture



Related Categories Cell Culture, Growth Factors and Cytokines, Hematopoietic Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   0.2-0.8 ng/mL ED50
quality   endotoxin tested
mol wt   monomer mol wt 24-30 kDa (glycosylated)
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/mg
suitability   suitable for cell culture
storage temp.   −20°C


Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.


10 μg in poly bottle

Biochem/physiol Actions

HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 

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