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H5416 Sigma-Aldrich

FLT3 Ligand human

recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, Hematopoietic Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   0.2-0.8 ng/mL ED50
quality   endotoxin tested
mol wt   monomer mol wt 24-30 kDa (glycosylated)
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/mg
suitability   suitable for cell culture
storage temp.   −20°C

Description

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human acute myeloid leukemia cell line OCI-AML5.

Packaging

10 μg in poly bottle

Biochem/physiol Actions

HumanKine FLT3 Ligand is expressed in human HEK 293 cells as a glycosylated monomer with an apparent molecular mass of 24-30 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

FLT3 ligand is a hematopoietic cytokine that regulates the proliferation of early hematopoietic cells. It has been shown to synergize with a variety of cytokines to stimulate the growth and differentiation of early hematopoietic cells. The FLT3 ligand also stimulates the expansion of monocytes and immature dendritic cells, and induces early B cell lineage differentiation and NK cell growth.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

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Peer-Reviewed Papers
15

References

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