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H5666 Sigma-Aldrich

Granulocyte-Macrophage Colony-Stimulating Factor human

GM-CSF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: GM-CSF human

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, Hematopoietic Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   0.2-2 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 15-36 kDa (glycosylated)
packaging   pkg of 5X10 μg
  pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/mg
suitability   suitable for cell culture
UniProt accession no.   P04141
storage temp.   −20°C
Gene Information   human ... CSF2(1437)

Description

Biochem/physiol Actions

Granulocyte-macrophage colony stimulating factor (GM-CSF) is a growth and differentiation factor for cells in the granulocyte, macrophage and eosinophil lineage. GM-CSF stimulates colony formation from pluripotential progenitor cells at extremely low concentrations and is an essential survival and proliferative factor for hematopoietic progenitor cells in all divisions up to maturity. It also stimulates growth in some epithelial cells and osteoclasts. GM-CSF is produced by a variety of cell types (monocytes, endothelial cells, T-cells, fibroblasts, mitogen-stimulated B-cells, and LPS-stimulated macrophages). GM-CSF is secreted as a single chain glycoprotein containing 128 amino acids for human with a conserved disulfide bond. Human and murine GM-CSF share approx. 54% sequence homology and do not cross-react in bioactivity.

Caution

Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

Preparation Note

HumanKine GM-CSF is expressed as a 15-36 kDa glycosylated monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications. Although human and mouse GM-CSF share 54% amino acid sequence homology, their biological actions are species-specific and exhibit no cross-species reactivity.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Hematopoietic Cytokines

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Keywords: Cell proliferation, Clinical, Growth factors, Ligands

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Peer-Reviewed Papers
15

References

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