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H5791 Sigma-Aldrich

Hepatocyte Growth Factor human

HGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: HGF, Hepatopoietin A, Scatter Factor

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, Hepatocyte Growth Factor/Scatter Factor, HumanKine Growth Factors and Cytokines, Reagents and Supplements More...
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   <100 ng/mL ED50/EC50
quality   endotoxin tested
mol wt   monomer 70 kDa (glycosylated)
packaging   pkg of 5x10 μg
  pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/mg
suitability   suitable for cell culture
UniProt accession no.   P14210
storage temp.   −20°C
Gene Information   human ... HGF(3082)

Description

General description

The HGF gene spans ∼70 kb and consists of 18 exons interrupted by 17 introns. The organization of the human HGF gene is highly homologous to that of human plasminogen. HGF maps to the long arm of human chromosome 7, 7q21.1.

Biochem/physiol Actions

HGF is a multifunctional cytokine that promotes mitogenesis, migration, invasion, and morphogenesis. It is a potent mitogen, stimulating the growth of hepatocytes, renal tubular epithelial cells, epidermal keratinocytes, epidermal melanocytes, Mv1Lu (mink lung epithelial cells), and BALB/MK (mouse keratinocytes). HGF inhibits the growth of B6/F1 (mouse melanoma) cells, KB (human squamous carcinoma) cells, and HepG2 (human hepatoma) cells. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Hepatocyte growth factor (HGF) is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene.

Other Notes

The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays.

Preparation Note

HumanKine HGF is expressed as a glycosylated 70 kDa single chain monomer in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the monkey epithelial cell line 4MBr-5.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Growth Factors in Stem Cell Biology

Stem cell biology researchers use suitable growth factors to trigger proliferation, differentiation and/or migration of stem cells. Embryonic pluripotent stem cells can differentiate into three germ ...
Keywords: Angiogenesis, Cell biology, Cell culture, Growth factors, Stem cell biology

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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