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H5912 Sigma-Aldrich

Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-H2AXS139p, H2Ax Antibody - Anti-phospho-Histone H2AX (pSer139) antibody produced in rabbit, H2Ax Antibody

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   microarray: suitable
  western blot: 1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine
species reactivity   mouse, human
mol wt   antigen mol wt 15 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P16104
Gene Information   human ... H2AFX(3014)
mouse ... H2afx(15270)

Description

General description

Histones (H1, H2B, H2A, H3 and H4) regulate the packaging of eukaryotic DNA into repeating nucleosome units that are folded in higher-order chromatin fibers . Phosphorylation of serine 139 in the histone H2AX subtype is induced by double-stranded breaks in DNA. H2AX histone has also been implicated in DNA repair mechanisms following double-strand breaks . Anti-phospho-Histone H2AX [pSer139] binds to histone H2AX phosphorylated on Ser139 (γ-H2AX) by immunoblotting (15 kDa). Staining of [pSer139] histone H2AX in immunoblotting is specifically inhibited with the [pSer139] histone H2AX immunizing peptide (human, amino acids 134-142). No inhibition is observed with the respective non-phosphorylated histone H2AX peptide (human, amino acids 134-142). The antibody product is also reactive in mice.

Immunogen

The synthetic peptide sequence is conjugated to KLH. The immunogen sequence is highly conserved (single amino acid substitution) in mouse histone H2AX.

synthetic phosphorylated peptide corresponding to the C-terminus (amino acids 134-142) of human histone H2AX (pSer139).

Application

Anti-phospho-Histone H2AX (p(Ser139M)) antibody is suitable for use in western blot (1:1,000 using whole cell extracts of HeLa (human epitheloid carcinoma) cells or NIH3T3 mouse fibroblast cells treated with staurosporine) and microarray. The antibody may also be used in immunoblot assays.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

200 μL in glass insert

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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