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H6147 Sigma-Aldrich

HEPES

BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895731

  •  NACRES NA.75

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Properties

Related Categories Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Cell Culture, Mouse Embryo Culture,
Quality Level   400
product line   BioXtra
assay   ≥99.5% (titration)
form   powder
application(s)   cell culture | embryo: suitable
pH   5.0-6.5 (25 °C, 238 g/L)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
  7.5
solubility   water: 500 mg/mL, clear, colorless
absorption   ≤0.05 at 290 in H2O at 33%
SMILES string   OCCN1CCN(CC1)CCS(O)(=O)=O
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key   JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg. It is typically used in cell culture at concentration between 5mM to 30mM.

Application

HEPES has been used:
• As a constituent of modified Eagle’s minimum essential medium for in vitro culturing of spermatogenic cells and as a constituent of the cryoprotectant used to preserve these germ cells
• As a component of modified Ham’s F-10 for the incubation of doe zygotes to carry out Haploid male hemizygous recipient production
• in the preparation of 90% Percoll solution used in Percoll- gradient method for sperm collection and preparation

Packaging

100, 500 g in poly bottle

Other Notes

This product has been qualified for use in cell and mouse embryo culture.

Easily compare specifications for HEPES products with the HEPES specification table.

Physical properties

Useful pH range: 6.8 - 8.2

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
TL6809000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

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Peer-Reviewed Papers
15

References

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