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H6254 Sigma-Aldrich

Hyaluronidase from sheep testes

Type V, lyophilized powder, ≥1,500 units/mg solid

Synonym: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase

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Description

General description

Hyaluronidase is present in high amount in testis as it aids fertilization of the mammalian egg. Mammalian hyaluronidase has a catalytic epidermal growth factor (EGF)-like domain and a C-terminal cysteine rich region.

Application

Hyaluronidase has been used:
• as a component of enzyme mix for the isolation of sertoli and germ cells from seminiferous tubules
• in the pre-treatment of deparaffinized adenomas sections for immunohistochemistry
• in the digestion of umbilical cord blood vessel for the generation of umbilical cord stromal stem cell lines

Packaging

1 g in poly bottle

500 mg in poly bottle

Biochem/physiol Actions

Hyaluronidase from sheep testis is commercially used to depolymerise hyaluronic acid and chondroitin-sulphate in connective tissue and cartilage respectively.

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will cause a change in % transmittance at 600 nm of 0.330 per minute at pH 5.35 at 37 °C in a 2.0 mL reaction mixture (45 minute assay).

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
Vicki Caligur
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatography, Coagulation, Degradations, Enzyme-linked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, Help, High performance liquid chromatography, Inflammation, Ion-exchange chromatography, Ligands, Mass spectrometry, Methods, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain reaction, Polymerization reactions, Proteomics, Reductive aminations, Sequences, Spectroscopy, Sulfations, Transcription, Type, transformation

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure is a turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) of Hyaluronidase activity. It is based on the following reaction:
Keywords: Extinction coefficient

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

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