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H6291 Sigma-Aldrich

Interleukin-1β human

IL-1β, recombinant, expressed in HEK 293 cells, HumanKine®, cell culture tested, endotoxin tested

Synonym: IL-1β, Lymphocyte activating factor

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Interleukin-1, Interleukins (IL),
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   ≤0.16 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 18-25 kDa (glycosylated)
packaging   pkg of 10 μg
impurities   <1 EU/μg
suitability   cell culture tested
UniProt accession no.   P01584
storage temp.   −20°C
Gene Information   human ... IL1B(3553)

Description

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse D10S cells.

Preparation Note

HumanKine Recombinant Human IL-1β, expressed in human HEK 293 cells, is a glycosylated monomer with a molecular mass of 18-25 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Biochem/physiol Actions

Interleukin-1 (IL-1), originally known as Lymphocyte Activating Factor (LAF), activates T cell lymphocytes, which then proliferate and secrete interleukin-2 (IL-2). IL-1 is released primarily from stimulated macrophages and monocytes, but has also been shown to be released from several other types and is thought to play a key role in inflammatory and immune responses. Other synonyms for IL-1 include: endogenous pyrogen (EP), mitogenic protein (MP), Helper Peak-1 (HP-1), T Cell Replacing Factor III (TRF III or TRFM), B Cell Activating Factor (BAF) and B Cell Differentiation Factor (BDF) The two closely related agents Interleukin-1α (IL-1α) and Interleukin-1β (IL-1β) share 62% homology in amino acid sequence and elicit nearly identical biological responses. IL-1α and IL-1β are both approximately 17 kDa with some heterogeneity in the amount of glycosylation.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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