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H6537 Sigma-Aldrich

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse

~1 mg/mL, clone HDAC3-83, purified immunoglobulin, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Epigenetics,
conjugate   unconjugated
clone   HDAC3-83, monoclonal
biological source   mouse
application(s)   microarray: suitable
  western blot: 1 μg/mL using total cell extracts of NIH3T3 cells
species reactivity   mouse, human
mol wt   antigen ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgM
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   O15379
Gene Information   human ... HDAC3(8841)
mouse ... Hdac3(15183)

Description

General description

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) (mouse IgM isotype) is derived from the HDAC3-83 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. The histone deacetylase 3 (HDAC3) gene is located on the human chromosome at 5q31.3. HDAC3 protein belongs to the class I HDAC.

Specificity

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) recognizes human and mouse HDAC3 (approx. 50 kDa).

Immunogen

synthetic peptide corresponding to amino acids 411-428 of human and mouse HDAC3.

Application

Monoclonal Anti-Histone Deacetylase 3 (HDAC3) antibody produced in mouse may be used in enzyme-linked immunosorbent assay (ELISA) and immunoblotting.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots at −20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Histone deacetylase 3 (HDAC3) protein is required for DNA replication, maintaining chromatin structure, genomic stability, and DNA damage control. This protein plays a role in eliminating histone acetylation. HDAC3 protein co-works with nuclear hormone corepressors, such as nuclear receptor corepressor (NCOR) and silencing mediator of retinoic acid and thyroid hormone receptor (SMRT). HDAC3 protein levels are high in proliferating cells of hepatocellular carcinoma.

Safety & Documentation

Safety Information

WGK Germany 
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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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