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H6666 Sigma-Aldrich

Keratinocyte Growth Factor human

KGF, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: FGF-7, KGF

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥90% (SDS-PAGE)
form   lyophilized powder
potency   ≤80 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 17-30 kDa (glycosylated)
packaging   pkg of 10 μg
mfr. no.   HumanZyme
impurities   <1 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P21781
storage temp.   −20°C
Gene Information   human ... FGF7(2252)

Description

Packaging

10 μg in glass bottle

Biochem/physiol Actions

KGF is a member of the fibroblast growth factor family. KGF is an epithelial cell specific mitogen responsible for the normal proliferation and differentiation of human epithelial cells. KGF is secreted in culture by stromal fibroblasts derived from major epithelial organs including the skin and gastrointestinal tract; and is expressed in vivo by dermal, but not epidermal cells. KGF transcripts are found in dermal fibroblasts, epidermal melanocytes, and malignant melanoma cells. KGF is particularly active as a mitogen for BALB/MK cells, a continuous mouse keratinocyte line, and as a potent mitogen, equivalent to EGF, for human keratinocytes in culture.

Preparation Note

HumanKine Keratinocyte Growth Factor (KGF) is expressed as glycosylated 17 and 30 kDa monomers in human HEK 293 cells. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of 4MBr-5 cells (monkey epithelial cell line).

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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K1757 Keratinocyte Growth Factor human, KGF, recombinant, expressed in E. coli, powder, suitable for cell culture

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