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H6909 Sigma-Aldrich

Hydrocortisone solution

50 μM, sterile-filtered, BioXtra, suitable for cell culture

Synonym: 17-Hydroxycorticosterone, Cortisol



Related Categories Cell Culture, Hormones, Reagents and Supplements More...
Quality Level   500
biological source   non-animal source
sterility   sterile-filtered
product line   BioXtra
form   liquid
concentration   50 μM
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
pH   5.0- 7.0
shipped in   dry ice
storage temp.   −20°C
SMILES string   C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@](O)(C(=O)CO)[C@@]4(C)C[C@H](O)[C@H]23
InChI   1S/C21H30O5/c1-19-7-5-13(23)9-12(19)3-4-14-15-6-8-21(26,17(25)11-22)20(15,2)10-16(24)18(14)19/h9,14-16,18,22,24,26H,3-8,10-11H2,1-2H3/t14-,15-,16-,18+,19-,20-,21-/m0/s1
Gene Information   human ... ABCB1(5243), CYP3A4(1576), NR3C1(2908), SERPINA6(866)
mouse ... Abcb1a(18671), Abcb1b(18669), Ifng(15978), Nos2(18126)
rat ... Ar(24208), Nr3c1(24413)



Hydrocortisone has been used in epithelial and endothelial adherent cell culture applications. Hydrocortisone solution has been used for the isolation of epidermal stem cells and cell expansion. It has also been used for keratinocyte permeability assay.

Other Notes

Animal-component free

Physical form

frozen in working aliquots, avoid repeated freeze/thaw

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
What is the concentration of Product No. H6909, Hydrocortisone solution?
Product No. H6909 is a 50 μM solution. This may also be written as approximately 0.0181 g/L.
Is Product No. H6909, Hydrocortisone solution, isotonic?
Product No. H6909 is a solution of hydrocortisone in normal saline (0.85%).It is an isotonic solution of hydrocortisone.
Is there ethanol in Product No. H6909, Hydrocortisone solution?
Hydrocortisone is initially solubilized in ethanol and then diluted in normal saline.The final concentration of ethanol is 2 ml/L (0.2% v/v).
Should Product No. H6909, Hydrocortisone solution, be protected from light?
Yes. Product No. H6909 is packaged in an amber vial.If making single use aliquots, we recommend to use amber vials to protect the solution from light.
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