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H7017 Sigma-Aldrich

Hemocyanin from Megathura crenulata (keyhole limpet)

Synonym: KLH, Keyhole limpet hemocyanin

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Properties

Related Categories Adjuvant Reagents, Antibodies, Antibody Purification and Characterization, Application Index, Biochemicals and Reagents,
Quality Level   300
biological source   Megathura crenulata
form   powder
packaging   vial of KLH (KLH is present by weight at approximately 1 part KLH to 5 parts total solid.)
application(s)   ELISA: suitable
solubility   H2O: soluble
storage temp.   2-8°C

Description

General description

Gastropod hemocyanins are massive glycoproteins (4 to 8 MDa) designed by an intricate arrangement of 10 subunits that are self-assembled into hollow cylinders 35 nm in diameter.

Hemocyanin is a high molecular weight copper containing glycoprotein. It reversibly binds oxygen and forms the extra-cellular respiratory protein of mollusks. Keyhole limpet hemocyanin (KLH) is filtered from the hemolymph of Megathura crenulata, also called the giant keyhole limpet. It is native to the southern California coast and Mexico.

Application

Keyhole limpet hemocyanin (KLH) is a strong and well known immune-stimulant in both experimental animals and humans. It has been used as a hapten carrier protein to enhance antigen-specific T cell priming and is known to stimulate a CD4+ T-cell response. Hemocyanins stimulate Th1 reactions without known side effects, which make them ideal for long-term continuous treatment of cancer.

Physical form

Lyophilized with stabilizing buffer

Reconstitution

Reconstitute with deionized water to a KLH concentration of 10 mg/mL, to yield an opalescent solution in 31 mM sodium phosphate buffer, pH 7.4, containing 0.46 M NaCl, 2% PVP and 41 mM sucrose.
Reconstituted protein solution may be stored up to 2 months at −20 °C.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

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HPLC Analysis of Proteins on SRT® SEC-2000, 30 cm x 7.8 mm I.D.

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Peer-Reviewed Papers
15

References

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