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  • H7033 - PerfectHyb Plus Hybridization Buffer

H7033 Sigma-Aldrich

PerfectHyb Plus Hybridization Buffer

for Northern and Southern blotting, solution

  •  NACRES NA.25



General description

Sigma’s PerfectHyb Plus is a ready-to-use hybridization buffer for Northern and Southern blot protocols. Use of PerfectHyb Plus can decrease hybridization time to as little as two hours.


Suitable for use in hybridization buffers used in Northern blot, Southern blot, colony/plaque blots, and dot blots.

Features and Benefits

• Fast and easy-to-use
• Up to 20x more sensitive than conventional buffers
• Doesn’t require blocking steps
• Compatible with neutral and positively-charged membranes
• Compatible with chemiluminescent, colorimetric , and DIG detection assays
• Compatible with radioactive and non-radioactive probes
• Compatible with single or double-stranded DNA, RNA, and oligonucleotide probes

Other Notes

A small amount of precipitate that has formed in the solution can be dissolved at 30-35°C with occasional gentle inversion.

Legal Information

PerfectHyb is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography


PerfectHyb™ Plus

Product Description Precautions and Disclaimer Storage/Stability Preparation Instructions Procedure Troubleshooting Guide Materials Reference
Keywords: AGE, Buffers, Electrophoresis, Gel electrophoresis, Molecular biology, Northern blot, Nucleic acid denaturation, Nucleic acid hybridization, Polymerase chain reaction, Southern blot

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Peer-Reviewed Papers


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