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H7041 Sigma-Aldrich

Interleukin-2 human

IL-2, recombinant, expressed in HEK 293 cells, cell culture tested, endotoxin tested

Synonym: Interleukin-2, T-cell growth factor

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, Interleukin-2, Interleukins (IL), Reagents and Supplements More...
biological source   human
recombinant   expressed in HEK 293 cells
form   lyophilized powder
potency   ≤5.0 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 15 kDa (glycosylated)
packaging   pkg of 5X10 μg
  pkg of 10 μg
  pkg of 100 μg
suitability   cell culture tested
UniProt accession no.   P60568
storage temp.   −20°C
Gene Information   human ... IL2(3558)

Description

General description

Interleukin-2 (IL-2) is an α-helical cytokine belonging to T-helper cell type 1. It has a molecular weight of 15kDa. The IL2 gene is mapped to human chromosome 4q27. IL-2 is originally called as T cell growth factor.

Application

Interleukin-2 human has been used to culture peripheral blood mononuclear cells (PBMC). It has been used to activate effector cell (PBMC) and to study antibody-dependent cell-mediated cytotoxicity.

Packaging

10 μg in poly bottle

Biochem/physiol Actions

Interleukin-2 (IL-2) is essential for proliferation of natural killer (NK) cells, lymphocytes and macrophages. It plays a key role in the immune system functions such as NK cell-stimulated immune responses. IL-2 also mediates the formation and essential functions of regulatory T cells. It is implicated in autoimmune disease. IL-2 is associated with apoptosis and thus, possesses antitumor response. IL-2 mediates CD41+ T-cells differentiation into T-helper cell 1 and 2 effector subsets, preventing T-helper 17 differentiation.

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

Preparation Note

Human Interleukin-2 (IL-2) expressed in human HEK 293 cells is a glycosylated monomer with a molecular mass of 15 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of mouse CTLL-2 cells (mouse cytotoxic T cell line).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Peer-Reviewed Papers
15

References

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