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H7140 Sigma-Aldrich

Hybridization Solution

for Northern and Southern blotting, liquid (2x concentrate)



Related Categories Buffer / Buffer Salts, Buffers for Hybridization, Core Bioreagents, Life Science Reagents for Northern and Southern Blotting, Molecular Biology,
form   liquid (2x concentrate)
foreign activity   DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected
storage temp.   −20°C


General description

Sigma’s Hybridization Solution is a 2x ready-to-use hybridization buffer for Northern and Southern blot protocols.


Hybridization Solution has been used to hybridise the nitrocellulose membrane. It has also been used in DNA hybridization.

Suitable for use as a hybridization buffer in Northern and Southern blot protocols.


Sigma’s Hybridization Solution contains 200 μg/mL DNA in 10x SSC and 2X Denhardt’s solutions:
200 μg/mL DNA
2mg Bovine Serum Albumin (BSA)
2mg Ficoll
2mg Polyvinylpyrrolidone (PVP)
0.15 M sodium citrate
1.5 M NaCl

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Protocols & Articles


Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Peer-Reviewed Papers


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GERPN3005 ECL Direct Nucleic Acid, GE Healthcare, RPN3005, pack of 1 ea
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