H7166 Sigma-Aldrich

Interleukin-3 human

IL-3, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: IL-3



Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Interleukin-3, Interleukins (IL),
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥90% (SDS-PAGE)
form   lyophilized powder
potency   0.5-2 ng/mL ED50
quality   endotoxin tested
mol wt   dimer mol wt 17-45 kDa (glycosylated)
packaging   pkg of 10 μg
mfr. no.   HumanZyme
impurities   <1 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P08700
storage temp.   −20°C
Gene Information   human ... IL3(3562)


Analysis Note

The activity was determined by the dosedependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).


10 μg in poly bottle

Preparation Note

HumanKine Interleukin-3 (IL-3), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 17-45 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications.

Biochem/physiol Actions

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

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