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H7291 Sigma-Aldrich

Interleukin-4 human

potency:, IL-4, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Interleukin-4, Interleukins (IL),
biological source   human
recombinant   expressed in HEK 293 cells
form   lyophilized powder
potency   0.1-0.5 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 14-19 kDa (glycosylated)
packaging   pkg of 1 mg
  pkg of 10 μg
  pkg of 100 μg
mfr. no.   HumanZyme
impurities   <1 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P05112
storage temp.   −20°C
Gene Information   human ... IL4(3565)

Description

Packaging

10 μg in poly bottle

Biochem/physiol Actions

IL-4 is a complex glycoprotein released by a subset of activated T cells, mast cells, and basophils. Human and mouse IL-4 share a 50% amino acid sequence homology, but their biological actions are species specific. IL-4 is a pleiotropic cytokine that has profound effects on the growth and differentiation of immunologically competent cells. IL-4 regulates differentiation of naïve CD4+ T cells to the TH2 type. IL-4 also regulates IgE and IgG1 production by B cells.

Preparation Note

HumanKine Interleukin-4 (IL-4), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 14-19 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Peer-Reviewed Papers
15

References

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