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H7291 Sigma-Aldrich

Interleukin-4 human

IL-4, potency:, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Interleukin-4, Interleukins (IL),
Quality Level   100
biological source   human
recombinant   expressed in HEK 293 cells
form   lyophilized powder
potency   0.1-0.5 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 14-19 kDa (glycosylated)
packaging   pkg of 1 mg
  pkg of 10 μg
  pkg of 100 μg
mfr. no.   HumanZyme
application(s)   cell culture | mammalian: suitable
impurities   <1 EU/μg
UniProt accession no.   P05112
storage temp.   −20°C
Gene Information   human ... IL4(3565)

Description

General description

Interleukin-4 (IL-4) is a pleiotropic cytokine and a complex glycoprotein mapped to human chromosome 5q31.1. It is produced by mast cells, basophils and a subset of activated T cells.

Packaging

10 μg in poly bottle

Biochem/physiol Actions

Interleukin-4 (IL-4) interacts with interleukin 13 and is an interleukin-4 receptor ligand. It regulates IgE and IgG1 production by B cells. IL-4 favors glial and neuronal differentiation and regulates differentiation of naive cluster of differentiation CD4+ T cells to the T helper type 2 (TH2) cells. Polymorphism in the IL-4 gene is implicated in immune and inflammatory disorders. In cerebral infarction, IL-4 levels are elevated.

Preparation Note

HumanKine Interleukin-4 (IL-4), expressed in human HEK 293 cells, is a glycosylated monomer with an apparent molecular mass of 14-19 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent simulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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