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H7416 Sigma-Aldrich

Interleukin-6 human

IL-6, recombinant, expressed in HEK 293 cells, suitable for cell culture

Synonym: IL-6

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Properties

Related Categories CNTF Family - includes ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), Cell Culture, Growth Factors and Cytokines, Interleukin-6, Interleukins (IL),
Quality Level   100
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   ≤2.0 ng/mL ED50
quality   endotoxin tested
mol wt   dimer 26-30 kDa (glycosylated)
packaging   pkg of 10 μg
application(s)   cell culture | mammalian: suitable
impurities   ≤1.00 EU/μg
UniProt accession no.   P05231
storage temp.   −20°C
Gene Information   human ... IL6(3569)

Description

General description

The IL6 (interleukin-6) gene is mapped to human chromosome 7p15.3. All the members of IL6 family have membrane glycoprotein gp130 as a common receptor. IL6 (interleukin-6) induces B-cell differentiation and is a primary regulator of inflammation.

Application

Interleukin-6 human has been used to check its role in breast cancer metastasis. It has also been used for evaluating its role in D5HS tumor sphere formation.

Packaging

10 μg in poly bottle

Biochem/physiol Actions

IL6 regulates tumor proliferation and inhibits apoptosis. It contributes to the disease progression of coronary artery disease.

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Preparation Note

Human IL-6, expressed in human 293 cells, is a glycosylated monomer with an apparent molecular mass of 26-30 kDa due to glycosylation, which is absent when this cytokine is expressed in E. coli. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

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Peer-Reviewed Papers
15

References

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