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H7523 Sigma-Aldrich

HEPES

BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Synonym: 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

  • CAS Number 7365-45-9

  • Empirical Formula (Hill Notation) C8H18N2O4S

  • Molecular Weight 238.30

  •  Beilstein/REAXYS Number 883043

  •  EC Number 230-907-9

  •  MDL number MFCD00006158

  •  PubChem Substance ID 24895796

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Properties

Related Categories BioXtra Buffers, Biochemicals and Reagents, Biological Buffers, Buffers A to Z, Good’s Buffers More...
Quality Level   400
product line   BioXtra
assay   ≥99.5% (titration)
form   powder
impurities   Insoluble matter, passes filter test
ign. residue (900°C)   ≤0.1% (as SO4)
loss   ≤1.0% loss on drying, 110°C
pH   5.0-6.5 (1 M in H2O)
useful pH range   6.8 - 8.2
pKa (25 °C)   7.5
solubility   H2O: 1 M, clear, colorless
anion traces   chloride (Cl-): ≤0.05%
cation traces   Al: ≤0.0005%
  As: ≤0.0001%
  Ba: ≤0.0005%
  Bi: ≤0.0005%
  Ca: ≤0.001%
  Cd: ≤0.0005%
  Co: ≤0.0005%
  Cr: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Li: ≤0.0005%
  Mg: ≤0.0005%
  Mn: ≤0.0005%
  Mo: ≤0.0005%
  Na: ≤0.005%
  Ni: ≤0.0005%
  Pb: ≤0.0005%
  Sr: ≤0.0005%
  Zn: ≤0.0005%
absorption   ≤0.035 at 280 in H2O at 1 M
  ≤0.045 at 260 in H2O at 1 M
Featured Industry   Diagnostic Assay Manufacturing
SMILES string   OCCN1CCN(CC1)CCS(O)(=O)=O
InChI   1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key   JKMHFZQWWAIEOD-UHFFFAOYSA-N

Description

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used:
• As a component of homogenisation buffer used in protein sample preparation
• To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
• As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
• As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.

Packaging

50, 250 g in poly bottle

Other Notes

Easily compare specifications for HEPES products with the HEPES specification table.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
RTECS 
TL6809000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Buffers - From Bnech to Bulk

pHast Pack
Protocols & Articles

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Peer-Reviewed Papers
15

References

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