EMAIL THIS PAGE TO A FRIEND
H7541 Sigma-Aldrich

Interleukin-10 human

IL-10, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: IL-10

Purchase

Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Interleukin-10, Interleukins (IL),
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   ≤3.0 ng/mL ED50
quality   endotoxin tested
mol wt   dimer mol wt 17 kDa (glycosylated)
impurities   ≤1EU/mg
suitability   suitable for cell culture
UniProt accession no.   P22301
storage temp.   −20°C
Gene Information   human ... IL10(3586)

Description

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of MC/9 cells (mouse master cell line) in presence of 200 pg/mL IL-4.

Packaging

10 μg in poly bottle

Preparation Note

HumanKine Interleukin-10 (IL-10) is expressed in human HEK 293 cells as a glycosylated, non-disulfide linked homodimer with an apparent molecular mass of 17 kDa due to glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Biochem/physiol Actions

IL-10 is an important regulator of the functions of lymphoid and myeloid cells. IL-10 can block the activation of cytokine synthesis and several accessory functions of macrophages. Human and mouse IL-10 share a 73% sequence homology. However, human IL-10 acts on both human and mouse target cells, while mouse IL-10 has species-specific activity. In mouse, the cellular sources of IL-10 consist of Th2 cells, activated fetal thymocytes, macrophages, keratinocytes, LY-1+ (CD5+), and normal B cells. In human, the cellular sources of IL-10 consist of CD4+ T cells and T cell clones, thymocytes, B cells, B cell lymphomas, macrophages, mast cell lines and keratinocytes. IL-10 stimulates the growth of stem cells, mast cells and thymocytes. IL-10 enhances cytotoxic T cell development, and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing the cell-type dependent expression of either angiogenic or angiostatic factors.

Interleukin-10 regulates lymphoid and myeloid cell functions. It blocks the activation of cytokine synthesis and several accessory functions of macrophages. IL-10 enhances cytotoxic T cell development and co-stimulates B cell differentiation and Ig secretion. IL-10 regulates angiogenesis by inducing cell-type dependent expression of either angiogenic or angiostatic factors.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?