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H7630 Sigma-Aldrich

Hyaluronic acid sodium salt from bovine vitreous humor

Synonym: Poly(β-glucuronic acid-[1→3]-β-N-acetylglucosamine-[1→4]), alternating

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Description

Packaging

10, 50 mg in poly bottle

Application

Hyaluronic acid sodium salt from bovine vitreous humor has been used:
• as a component for the generation of artificial synovial fluid
• to investigate its role in inducing oxidative stress and interleukin 8(IL-8) production in human nasal RPMI 2650 cells
• to test its effect on B cell proliferation
• as a substrate in neuraminidase assay of morbilliviruses virus

Biochem/physiol Actions

High molecular mass polymer composed of repeating dimeric units of glucuronic acid and N-acetyl glucosamine which forms the core of complex proteoglycan aggregates found in extracellular matrix.

Hyaluronic acid (HA) is abundantly present in the bovine vitreous humor. It corresponds to a molecular weight of 69293.9Da and has an isoelectric pH of 5.82. Bovine HA has high levels of endotoxins and it polydisperse with DNA and RNA contaminants. The enzyme HA synthases at the plasma membrane (HASs) aids in the synthesis of circulatory HA. HA is implicated in many physiological processes including inflammation, immune regulation, adhesion, embryo development and tumor progression.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
MT7250000
Protocols & Articles

Articles

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units. The disaccharide units that are the basis of all glycosaminoglycans (except keratan) are a combination...
Vicki Caligur
BioFiles 2008, 3.10, 4.
Keywords: Adhesion, Aminations, Angiogenesis, Anticoagulants, Apoptosis, Atomic absorption spectroscopy, Cancer, Cell biology, Cell proliferation, Cell signaling, Cellular processes, Central Nervous System, Chromatography, Coagulation, Degradations, Enzyme-linked immunosorbent assay, Epimerizations, Gene expression, Genetic, Genomics, Growth factors, Help, High performance liquid chromatography, Inflammation, Ion-exchange chromatography, Ligands, Mass spectrometry, Methods, Microarray Analysis, Morphogenesis, Nuclear magnetic resonance spectroscopy, Phosphorylations, Polymerase chain reaction, Polymerization reactions, Proteomics, Reductive aminations, Sequences, Spectroscopy, Sulfations, Transcription, Type, transformation

Glycosaminoglycans and Proteoglycans

Glycosaminoglycans (GAGs) are large linear polysaccharides constructed of repeating disaccharide units with the primary configurations containing an amino sugar (either GlcNAc or GalNAc) and an uroni...
Glycobiology Analysis Manual, 2nd Edition
Keywords: Adhesion, Catalysis, Coagulation, Degradations, Growth factors, Inflammation, Ligands, Pentose phosphate pathway, Polymerization reactions, Sulfations

Protocols

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure is a turbidimetric determination (% Transmittance at 600 nm, Light path = 1 cm) of Hyaluronidase activity. It is based on the following reaction:
Keywords: Extinction coefficient

Peer-Reviewed Papers
15

References

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