• USA Home
  • H8163 - Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
H8163 Sigma-Aldrich

Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-HDAC5

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Epigenetics,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: 1-2 μg/mL using cultured NIH-3T3 or HeLa cells
  immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin-embedded human breast carcinoma section
  immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant mouse HDAC5
  microarray: suitable
  western blot (chemiluminescent): 2-4 μg/mL using whole extract of human embryonal kidney 293T cells and whole extract of rat pheochromocytoma PC-12 cells
species reactivity   rat, mouse, human
mol wt   antigen ~124 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q9UQL6
Gene Information   human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)

Description

Immunogen

synthetic peptide corresponding to amino acid residues 384-399 of human HDAC5 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?