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  • H8163 - Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

H8163 Sigma-Aldrich

Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-HDAC5



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Epigenetics,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: 1-2 μg/mL using cultured NIH-3T3 or HeLa cells
  immunohistochemistry: 5-10 μg/mL using formalin-fixed paraffin-embedded human breast carcinoma section
  immunoprecipitation (IP): 1-2 μg using extract of 293T cells expressing recombinant mouse HDAC5
  microarray: suitable
  western blot (chemiluminescent): 2-4 μg/mL using whole extract of human embryonal kidney 293T cells and whole extract of rat pheochromocytoma PC-12 cells
species reactivity   rat, mouse, human
mol wt   antigen ~124 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q9UQL6
Gene Information   human ... HDAC5(10014)
mouse ... Hdac5(15184)
rat ... Hdac5(84580)


General description

Histone Deacetylase 5 (HDAC5) belongs to the class IIa HDAC family. The localization of HDAC5 is both nuclear and cytoplasmic. HDAC5 gene is mapped to human chromosome at 17q21.31.


Anti-Histone Deacetylase 5 (HDAC5)(NA-16) recognizes human, rat, and mouse HDAC5.


synthetic peptide corresponding to amino acid residues 384-399 of human HDAC5 with N-terminal added cysteine, conjugated to KLH. The corresponding sequence is identical in rat and mouse.


Anti-Histone Deacetylase 5 (HDAC5) (NA-16) antibody produced in rabbit has been used in:
• chromatin immunoprecipitation
• immunostaining
• immunocytochemistry
• immunoprecipitation

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in frost-free freezers,is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Histone Deacetylase 5 (HDAC5) shuttling to the cytoplasm occurs during myogenic differentiation, the nuclear export being stimulated by calcium/ calmodulin-dependent protein kinase (CaMK) phosphorylation at Ser259 and Ser498. HDAC5 activity is important for the differentiation of muscle cells by binding, through its N-terminal domain, to the myocyte enhancer factor-2 (MEF2) protein, thus repressing expression of MEF2 downstream genes.Overexpression of HDAC5 in various cancer cells suppresses their growth by induction of apoptosis in a p53-independent manner. It also plays a key role in many biological processes including the synoviocyte activation, neural regeneration and repair. HDAC5 is highly expressed in breast cancer and contributes to tumor progression.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles


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Peer-Reviewed Papers


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