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  • H8289 - Anti-dimethyl-Histone H1.4 (diMe-Lys26) antibody produced in rabbit

H8289 Sigma-Aldrich

Anti-dimethyl-Histone H1.4 (diMe-Lys26) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym: Anti-H1.4K26me2, Anti-H1K26me2



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5-1 μg/mL using acid-extracted fraction of HL60 cells.
species reactivity   human
mol wt   antigen mol wt ~35 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~1.5 mg/mL
UniProt accession no.   P10412
Gene Information   human ... HIST1H1E(3008)



ChIP validated


dimethylated synthetic peptide corresponding to amino acids 22-23 (diMeLys26) of human histone H1.4.


Anti-dimethyl-Histone H1.4 (diMe-Lys26) antibody produced in rabbit is suitable for western blotting at a concentration of 0.5-1μg/mL using acid-extracted fraction of HL60 cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The building block of chromatin is the nucleosome which is comprised of an octamer of core histones (H2A, H2B, H3, and H4) around which 147 bp of DNA are wrapped. In mammalian cells, four histone H1 variants (H1.2 to H1.5) are present in all somatic cells, and a fifth (H1.1) is restricted to thymus, testis, and spleen and possibly lymphocytic and neuronal cells. Histone H1.4 is di-methylated or acetylated at Lys26. Lys26 is located within the flexible N-terminal domain of H1.4, just preceding the globular domain.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers


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H7789 Anti-acetyl-Histone H1.4 (Ac-Lys26) antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
H8164 Anti-dimethyl, phospho-Histone H1.4 (diMe-Lys26, pSer27) antibody produced in rabbit, ~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

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