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H8291 Sigma-Aldrich

Platelet-Derived Growth Factor-AA human

PDGF-AA, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: PDGF−AA

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Platelet Derived Growth Factors (PDGF), Reagents and Supplements More...
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   ≤2000 ng/mL Specific activity ED50
quality   endotoxin tested
mol wt   dimer 35-45 kDa (glycosylated)
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P04085
storage temp.   −20°C
Gene Information   human ... PDGFA(5154)

Description

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells.

Packaging

10 μg in poly bottle

Preparation Note

HumanKine recombinant human PDGF-AA, expressed in human HEK 293 cells, is a mixture of pro-form, pro-mature, and mature dimers with apparent molecular masses of 35, 40, and 45 kDa, respectively. They are disulfide linked glycosylated homodimers. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

Application

Platelet-Derived Growth Factor-AA human has been used in in vitro transdifferentiation of mesenchymal stem cells into SC (Schwann cells)-like cells.

Biochem/physiol Actions

PDGF (platelet-derived growth factor) not only functions as a growth factor, it also participates in chemotaxis for triggering cell movement. Elevated levels of placental PDGF is observed during late first and third trimester pregnancies. PDGF is associated with wound healing and atherosclerosis. PDGFA mediated PDGFRa (PDGF receptor a) phosphorylation activates pathways associated with cancer. PDGFA has been indicated to induce growth and metastasis in human hepatocellular carcinoma.
The sources of PDGF during wound repair include platelets (predominantly PDGF-AB), smooth muscles (PDGF-A),18 monocyte-derived macrophages (PDGF-B),19 and endothelial cells (PDGF- B).20 PDGF may play a role during normal embryonic development.
Purified PDGF activates two distinct PDGF receptors encoded by separate genes. PDGF-AA binds only to -PDGF receptor but PDGF-AB and PDGF-BB bind to both alpha and β-receptors; i.e., the alpha-receptor binds either A or B chain and the β-receptor binds only the B chain.

General description

PDGF (platelet-derived growth factor), is the principal serum mitogen predominantly found in cells of mesenchymal origin. It is expressed in smooth muscle cells, endothelial cells and macrophages. PDGF is localized in α-granules of platelets and released during clot formation. PDGF from human platelets has been purified and described as a cationic glycoprotein (pI 9.5-10.4) having a molecular mass of approx. 30 kDa and composed of two covalently linked subunits, designated as chains A (16 kDa) and B (14 kDa). PDGF exists as five different dimers : heterodimers occurs as PDGF-AB and homodimers exists as FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD). In platelets, approx.70% of the PDGF is present as the AB dimer, with most of the remainder as BB. Purified human PDGF shows substantial size heterogeneity, ranging from 27-31 kDa, probably due to the presence of isoforms, glycosylation processing, aging of the platelets, and partial proteolysis during purification.
The PDGF gene is highly conserved and is mapped to human chromosome 7p22.3. PDGF is structurally and functionally related to vascular endothelial growth factors.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
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Peer-Reviewed Papers
15

References

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