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H8541 Sigma-Aldrich

Transforming Growth Factor-β1 human

TGF-β1, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: TGF-β1

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements, Recombinant TGF proteins,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   0.1-0.5 ng/mL ED50
quality   endotoxin tested
mol wt   dimer mol wt 25 kDa (non-glycosylated)
packaging   pkg of 100 μg
  pkg of 5 μg
mfr. no.   HumanZyme
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P01137
storage temp.   −20°C
Gene Information   human ... TGFB1(7040)

Description

Analysis Note

The specific activity was determined by the dose dependent inhibition of IL-4 induced proliferation of mouse HT-2 cells (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Biochem/physiol Actions

Transforming growth factor-β1 (TGF-β1) is produced by many cell types, but is reported to be most concentrated in mammalian platelets, where it is present at approximately four times the level of TGF-β2.

TGF-β1 is a multifunctional peptide capable of influencing cell proliferation, differentiation, and other functions in a wide range of cell types. Transformed as well as non-neoplastic tissues release transforming growth factors and essentially all cells possess a specific TGF-β1 receptor. In general, cells of mesenchymal origin appear to be stimulated by TGF-β1; whereas, hepatocytes, T and B lymphocytes, keratinocytes, and many epithelial cells are inhibited by the peptide. TGF-β1 interacts with Epidermal Growth Factor (EGF), Platelet Derived Growth Factor (PDGF), Fibroblast Growth Factor (FGF), and T Cell Growth Factor (T-CGF) either by enhancing or antagonizing their characteristic actions. TGF-β1 plays a fundamental role in tissue growth and differentiation by involvement in adipogenesis, myogenesis, chondrogenesis, osteogenesis, epithelial cell differentiation, and immune cell function. TGF-β1 is associated with the developmental and metastasis of colorectal cancer.

Packaging

5 μg in poly bottle

Preparation Note

HumanKine TGF-β1 is expressed in human HEK 293 cells as a mature, disulfide linked, non- glycosylated, homodimer with a predicted molecular mass of 25 kDa.

Application

Transforming Growth Factor-β1 (TGF-β) human has been used :
• to study the expression of PDGFRB (Platelet derived growth factor receptor β) in association with transforming growth factor-β signaling.
• to stimulate lung fibrosis in human fetal lung fibroblast cell line in order to further measure the in-plane elasticity of live cell layers using a pressure sensor embedded microfluidic device.
• to study the effect of TGF-β on HTRA1 (high-temperature requirement A1)-affected Wnt target genes.

General description

The TGFB1 gene is mapped to human chromosome 19q13.2 and codes for a 25 kDa peptide. TGF-ß1 polypeptide is produced as a precursor with hydrophobic signal sequence, pro-region and mature peptide. Cleavage of the precursor at tetrabasic cleavage site results in monomers of carboxy-terminal 112 amino acids. The biologically active form of Tgfb1 exists as a disulphide-linked homodimer.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
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References

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